Journal: Medical Oncology (Northwood, London, England)

Article Title: Ashwagandha ( Withania somnifera ) targets liver cancer stem cells via inhibiting Hedgehog signaling pathway in hepatocellular carcinoma

doi: 10.1007/s12032-025-03215-0

Figure Lengend Snippet: Flow cytometry analysis of CD90 expression in HepG2 cells treated with SOR and/or ASH-AE for 48 h. *: Statistically significant difference from control group at p ≤ 0.05, #: Statistically significant difference from SOR group at p ≤ 0.05

Article Snippet: After 24 h, cells were treated with IC50 of SOR and/or ASH-AE in triplicates and incubated in the same conditions for 48 h. After that, cells were collected and 1 × 10 6 cells were resuspended in 100 μl PBS and then incubated with PE/Elab Fluor ® 594 Anti-Human CD90 Antibody (1:50 dilution) (Elabscience, USA) for 30 min at room temperature.

Techniques: Flow Cytometry, Expressing, Control